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Mice treated according to the NIAAA model of AH were given daily aspirin or vehicle. ( A ) Aspirin resulted in reduced liver steatosis. Scale bar: 110 μm. <t>Total</t> <t>RNA</t> was isolated for the liver and analyzed by bulk RNA-seq. ( B ) Volcano plot of differentially expressed genes. ( C and D ) Hallmark pathway analysis top significantly downregulated pathways in the aspirin groups, and GO-BP pathway analysis top significantly downregulated pathways in the aspirin group. Two-tailed unpaired t test for 2 groups with Welch’s correction was used. * P < 0.05. GO-BP, gene ontology biological process.
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Mice treated according to the NIAAA model of AH were given daily aspirin or vehicle. ( A ) Aspirin resulted in reduced liver steatosis. Scale bar: 110 μm. Total RNA was isolated for the liver and analyzed by bulk RNA-seq. ( B ) Volcano plot of differentially expressed genes. ( C and D ) Hallmark pathway analysis top significantly downregulated pathways in the aspirin groups, and GO-BP pathway analysis top significantly downregulated pathways in the aspirin group. Two-tailed unpaired t test for 2 groups with Welch’s correction was used. * P < 0.05. GO-BP, gene ontology biological process.

Journal: JCI Insight

Article Title: Platelet-derived S100A9 contributes to endotheliopathy in alcohol-associated hepatitis

doi: 10.1172/jci.insight.201114

Figure Lengend Snippet: Mice treated according to the NIAAA model of AH were given daily aspirin or vehicle. ( A ) Aspirin resulted in reduced liver steatosis. Scale bar: 110 μm. Total RNA was isolated for the liver and analyzed by bulk RNA-seq. ( B ) Volcano plot of differentially expressed genes. ( C and D ) Hallmark pathway analysis top significantly downregulated pathways in the aspirin groups, and GO-BP pathway analysis top significantly downregulated pathways in the aspirin group. Two-tailed unpaired t test for 2 groups with Welch’s correction was used. * P < 0.05. GO-BP, gene ontology biological process.

Article Snippet: Total RNA isolated from the livers of aspirin and PBS-treated mice was sent to Plasmidsaurus for sequencing using an Illumina NovaSeq X Plus (catalog 20084804).

Techniques: Isolation, RNA Sequencing, Two Tailed Test